Cilt 4, Sayı 1, Sayfalar 12 - 19 2017-12-21

MOTILITY EVALUATION AND CRYOPRESERVATION OF FISH SPERM EXPOSED BY WATER-BORNE AND FOOD-BORNE BORON

Burak Evren İnanan [1] , Fevzi Yılmaz [2]

12 25

Boron (B) is required for organisms as an essential nutrient, whereas could be toxic in definite doses. In this study, for the first time, the effects of water-borne and food-borne B on fish sperm motility were initially determined and these sperm samples were cryopreserved in liquid nitrogen aiming to reveal how the quality of these samples are affected by cryopreservation. 12 mature male goldfish (Carassius auratus) individuals for each treatment performed in triplicate for 45 days were used in the study. Fish were fed with diets containing 1 mg, 5 mg and 10 mg of B per kg feed for foodborne experiments. In waterborne experiments, fish were kept in aquariums containing 1 mg/L, 10 mg/L and 20 mg/L of B. In both fresh and cryopreserved samples, the motility durations (s) and percentages of the sperm samples, vitality of spermatozoa (%) were determined. In the fresh samples, percentages of sperm motility and viability in the both B treatments decreased compared to the control, while increasing B concentration. After cryopreservation, the sperm samples in 5 mg of B per kg feed treatment has shown higher duration of sperm motility. Therefore, it is shown that feed-borne B could improve sperm resistance to cold shock.

Boron,Carassius auratus,Sperm motility,Sperm vitality,Cryopreservation
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Orcid: 0000-0002-2888-8457
Yazar: Burak Evren İnanan
E-posta: burakinanan@aksaray.edu.tr
Kurum: Aksaray University, Eskil Vocational School, Department of Veterinary Science, Aksaray
Ülke: Turkey


Orcid: 0000-0001-7487-6652
Yazar: Fevzi Yılmaz
E-posta: yfevzi@mu.edu.tr
Kurum: Muğla Sıtkı Koçman University, Faculty of Science, Department of Biology, Muğla
Ülke: Turkey


Bibtex @araştırma makalesi { jaefr317729, journal = {Journal of Aquaculture Engineering and Fisheries Research}, issn = {}, address = {Özkan ÖZDEN}, year = {2017}, volume = {4}, pages = {12 - 19}, doi = {}, title = {MOTILITY EVALUATION AND CRYOPRESERVATION OF FISH SPERM EXPOSED BY WATER-BORNE AND FOOD-BORNE BORON}, language = {en}, key = {cite}, author = {Yılmaz, Fevzi and İnanan, Burak Evren} }
APA İnanan, B , Yılmaz, F . (2017). MOTILITY EVALUATION AND CRYOPRESERVATION OF FISH SPERM EXPOSED BY WATER-BORNE AND FOOD-BORNE BORON. Journal of Aquaculture Engineering and Fisheries Research, 4 (1), 12-19. Retrieved from http://jaefr.scientificwebjournals.com/issue/33185/317729
MLA İnanan, B , Yılmaz, F . "MOTILITY EVALUATION AND CRYOPRESERVATION OF FISH SPERM EXPOSED BY WATER-BORNE AND FOOD-BORNE BORON". Journal of Aquaculture Engineering and Fisheries Research 4 (2017): 12-19 <http://jaefr.scientificwebjournals.com/issue/33185/317729>
Chicago İnanan, B , Yılmaz, F . "MOTILITY EVALUATION AND CRYOPRESERVATION OF FISH SPERM EXPOSED BY WATER-BORNE AND FOOD-BORNE BORON". Journal of Aquaculture Engineering and Fisheries Research 4 (2017): 12-19
RIS TY - JOUR T1 - MOTILITY EVALUATION AND CRYOPRESERVATION OF FISH SPERM EXPOSED BY WATER-BORNE AND FOOD-BORNE BORON AU - Burak Evren İnanan , Fevzi Yılmaz Y1 - 2017 PY - 2017 N1 - DO - T2 - Journal of Aquaculture Engineering and Fisheries Research JF - Journal JO - JOR SP - 12 EP - 19 VL - 4 IS - 1 SN - -2149-0236 M3 - UR - Y2 - 2017 ER -
EndNote %0 Journal of Aquaculture Engineering and Fisheries Research MOTILITY EVALUATION AND CRYOPRESERVATION OF FISH SPERM EXPOSED BY WATER-BORNE AND FOOD-BORNE BORON %A Burak Evren İnanan , Fevzi Yılmaz %T MOTILITY EVALUATION AND CRYOPRESERVATION OF FISH SPERM EXPOSED BY WATER-BORNE AND FOOD-BORNE BORON %D 2017 %J Journal of Aquaculture Engineering and Fisheries Research %P -2149-0236 %V 4 %N 1 %R %U